Archaebacterial Citrate Synthases: The Enzymes from the Thermoacidophiles Sulfolobus acidocaldarius and Thermoplasma acidophilum Show pro-S Stereospecificity
نویسندگان
چکیده
Citrate synthase from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius was purified 365-fold to electrophoretic homogeneity. At 40 °C and pH 8.1 the homogeneous enzyme shows a specific activity of 73 units per mg, which corresponds to a turnover number of 44 sec-1. Citrate synthase from S. acidocaldarius shows pro-S stereospecificity, as is found with a partially purified preparation of the enzyme from Thermoplasma acidophilum, another thermoacidophilic archaebacterium.
منابع مشابه
TRNomics: a comparative analysis of Picrophilus torridus with other archaeal thermoacidophiles.
In the euryarchaeal thermoacidophile Picrophilus torridus DSM 9790, we identified a copy of rare tRNA(Ile)(TAT) gene, along with the other 47 tDNAs with the help of our in-house program. Further, tRNAs of P. torridus were also compared with other archaeal thermoacidophiles Thermoplasma acidophilum, T. volcanium, Sulfolobus solfataricus and S. tokodaii.
متن کاملPurification and characterization of glucose dehydrogenase from the thermoacidophilic archaebacterium Thermoplasma acidophilum.
Glucose dehydrogenase was purified to homogeneity from the thermoacidophilic archaebacterium Thermoplasma acidophilum. The enzyme is a tetramer of polypeptide chain Mr 38,000 +/- 3000, it is catalytically active with both NAD+ and NADP+ cofactors, and it is thermostable and remarkably resistant to a variety of organic solvents. The amino acid composition was determined and compared with those o...
متن کاملChemiosmotic energy conversion of the archaebacterial thermoacidophile Sulfolobus acidocaldarius: oxidative phosphorylation and the presence of an F0-related N,N'-dicyclohexylcarbodiimide-binding proteolipid.
The energy-transducing mechanism of the thermoacidophilic archaebacterium Sulfolobus acidocaldarius DSM 639 has been studied, addressing the question whether chemiosmotic proton gradients serve as an intermediate energy store driving an F0F1-analogous ATP synthase. At pH 3.5, respiring S. acidocaldarius cells developed an electrochemical potential of H+ ions, consisting mainly of a proton gradi...
متن کاملSequences of the 5S rRNAs of the thermo-acidophilic archaebacterium Sulfolobus solfataricus (Caldariella acidophila) and the thermophilic eubacteria Bacillus acidocaldarius and Thermus aquaticus.
We have determined the nucleotide sequences of the 5 S rRNAs of three thermophilic bacteria: the archaebacterium Sulfolobus solfataricus, also named Caldariella acidophila, and the eubacteria Bacillus acidocaldarius and Thermus aquaticus. A 5 S RNA sequence for the latter species had already been published, but it looked suspect on the basis of its alignment with other 5 S RNA sequences and its...
متن کاملCloning, expression, and characterization of cis-polyprenyl diphosphate synthase from the thermoacidophilic archaeon Sulfolobus acidocaldarius.
cis-polyprenyl diphosphate synthases are involved in the biosynthesis of the glycosyl carrier lipid in most organisms. However, only little is known about this enzyme of archaea. In this report, we isolated the gene of cis-polyprenyl diphosphate synthase from a thermoacidophilic archaeon, Sulfolobus acidocaldarius, and characterized the recombinant enzyme.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2013